Back Cover: Development of a highly sensitive imaged cIEF immunoassay for studying AAV capsid protein charge heterogeneity
نویسندگان
چکیده
DOI: 10.1002/elps.202300039 This cover image shows a highly sensitive imaged capillary isoelectric focusing (icIEF) immunoassay for studying adeno-associated virus (AAV) capsid protein charge heterogeneity. AAVs are denatured and loaded into the of Peggy Sue™ instrument. Separated proteins immobilized to wall immunodetection using antibodies targeting three (VP1, VP2, VP3). method combines resolving power icIEF with specificity, selectivity, sensitivity an support extended characterization in upstream process steps.
منابع مشابه
Development of a highly sensitive three-dimensional gel electrophoresis method for characterization of monoclonal protein heterogeneity.
Three-dimensional gel electrophoresis (3-DE), which combines agarose gel electrophoresis and isoelectric focusing/SDS-PAGE, was developed to characterize monoclonal proteins (M-proteins). However, the original 3-DE method has not been optimized and its specificity has not been demonstrated. The main goal of this study was to optimize the 3-DE procedure and then compare it with 2-DE. We develope...
متن کاملHighly Sensitive FRET-Based Fluorescence Immunoassay for Detecting of Aflatoxin B1 Using Magnetic/Silica Core-Shell as a Signal Intensifier
Background: Recently, some new nanobiosensors using different nanoparticles or microarray systems for detection of mycotoxins have been designed . However, rapid, sensitive and early detection of aflatoxicosis would be very helpful to distinguish high-risk persons. Objectives: We report a highly sensitive competitive immunoassay using magnetic/silica core shell as a signal intensifier for the d...
متن کاملAAV capsid CD8+ T-cell epitopes are highly conserved across AAV serotypes
Adeno-associated virus (AAV) has become one of the most promising vectors in gene transfer in the last 10 years with successful translation to clinical trials in humans and even market approval for a first gene therapy product in Europe. Administration to humans, however, revealed that adaptive immune responses against the vector capsid can present an obstacle to sustained transgene expression ...
متن کاملDevelopment of a simple and highly sensitive enzyme immunoassay for hepatitis C virus core antigen.
A highly sensitive enzyme immunoassay (EIA) for the hepatitis C virus (HCV) core antigen (HCVcAg) was developed, and its performance was compared with that of the AMPLICOR HCV test (Roche Molecular Systems). The developed one-step pretreatment method, 30-min incubation of the specimen with a solution containing three different types of detergents (Triton X-100, 3-[(3-cholamidopropyl)-dimethylam...
متن کاملExpansion of a highly sensitive and specific ELISA test for diagnosis of hydatidosis using recombinant EgB8/2 protein
Objective(s): Hydatidosis is a zoonotic infection and endemic in Iran. Due to the serological cross-reactivity (of sera) with other parasitic infection, diagnosis of hydatid cyst is considered to be problematic. In this regard, application of recombinant antigens improves serological diagnosis for human hydatidosis. Here, we present an ELISA test based on B8/2 recombinant antigen of Echinococcu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Electrophoresis
سال: 2023
ISSN: ['0173-0835', '1522-2683']
DOI: https://doi.org/10.1002/elps.202370085